Antioxidant flavonoid-loaded nano-bioactive glass bone paste: in vitro apatite formation and flow behavior.

Non-cement pastes in the form of injectable materials have gained considerable attention in non-invasive regenerative medicine. Different osteoconductive bioceramics have been used as the solid phase of these bone pastes. Mesoporous bioactive glass can be used as an alternative bioceramic for paste preparation because of its osteogenic qualities. Plant-derived osteogenic agents can also be used in paste formulation to improve osteogenesis; however, their side effects on physical and physicochemical properties should be investigated. In this study, nano-bioactive glass powder was synthesized by a sol-gel method, loaded with different amounts of quercetin (0, 100, 150, and 200 µM), an antioxidant flavonoid with osteogenesis capacity. The loaded powder was then homogenized with a mixture of hyaluronic acid and sodium alginate solution to form a paste. We subsequently evaluated the rheological behavior, injectability, washout resistance, and in vitro bioactivity of the quercetin-loaded pastes. The washout resistance was found to be more than 96% after 14 days of immersion in simulated body fluid (SBF) as well as tris-buffered and citric acid-buffered solutions at 25 °C and 37 °C. All pastes exhibited viscoelastic behavior, in which the elastic modulus exceeded the viscous modulus. The pastes displayed shear-thinning behavior, in which viscosity was more influenced by angular frequency when the quercetin content increased. Results indicated that injectability was much improved using quercetin and the injection force was in the range 20-150 N. Following 14 days of SBF soaking, the formation of a nano-structured apatite phase on the surfaces of quercetin-loaded pastes was confirmed through scanning electron microscopy, X-ray diffractometry, and Fourier-transform infrared spectroscopy. Overall, quercetin, an antioxidant flavonoid osteogenic agent, can be loaded onto the nano-bioactive glass/hyaluronic acid/sodium alginate paste system to enhance injectability, rheological properties, and bioactivity.

Investigating the effect of mesenchymal stem cells on the rate of clinical and pathological improvement of asthmatic lung in mouse model.

Asthma is a pulmonary disease and its pathophysiology includes inflammation, obstruction, edema of the airways, and mucus secretions in the airways. Mesenchymal stem cells (MSCs) are self-renewal that use the therapeutic potential of these cells can be applied as treatments of asthma. In this study, the effect of Mesenchyme stem cells on asthma was investigated. MSCs were administrated for asthmatic mice and then, percentage of eosinophils in blood and bronchoalveolar lavage fluid (BALF), levels of interleukine (IL)-4 and Immunoglubolin (Ig)E were measured. Also histopathological study of lung tissue was done. MSCs administration could control percentage of eosinophils in blood and BALF, levels of IgE and IL-4, eosinophilic inflammation, mucin realizing and goblet cell hyper-plasia. Administration of MSCs as treatment of asthma can be a useful and applicable therapy in control of asthma symptoms.

Design and Manufacture of Bone Cements Based on Calcium Sulfate Hemihydrate and Mg, Sr-Doped Bioactive Glass.

In the present study, a novel composite bone cement based on calcium sulfate hemihydrate (CSH) and Mg, Sr-containing bioactive glass (BG) as solid phase, and solution of chitosan as liquid phase were developed. The phase composition, morphology, setting time, injectability, viscosity, and cellular responses of the composites with various contents of BG (0, 10, 20, and 30 wt.%) were investigated. The pure calcium sulfate cement was set at approximately 180 min, whereas the setting time was drastically decreased to 6 min by replacing 30 wt.% glass powder for CSH in the cement solid phase. BG changed the microscopic morphology of the set cement and decreased the size and compaction of the precipitated gypsum phase. Replacing the CSH phase with BG increased injection force of the produced cement; however, all the cements were injected at a nearly constant force, lower than 20 N. The viscosity measurements in oscillatory mode determined the shear-thinning behavior of the pastes. Although the viscosity of the pastes increased with increasing BG content, it was influenced by the frequency extent. Pure calcium sulfate cement exhibited some transient cytotoxicity on human-derived bone mesenchymal stem cells and it was compensated by introducing BG phase. Moreover, BG improved the cell proliferation and mineralization of extracellular matrix as shown by calcein measurements. The results indicate the injectable composite cement comprising 70 wt.% CSH and 30 wt.% Mg, Sr-doped BG has better setting, mechanical and cellular behaviors and hence, is a potential candidate for bone repair, however more animal and human clinical evaluations are essential.

Nanocomposite chitosan dressing incorporating polydopamine­copper Janus nanoparticle.

This research aims to introduce a new wound dressing with antibacterial and anti-inflammatory properties made from chitosan and copper-containing Janus nanoparticles (JNPs). The JNPs were synthesized by attaching copper to PDA nanospheres, which were then embedded in Chitosan at different concentrations. The resulting spherical JNPs had a mean size of 208 ± 96 nm, and EDX mapping showed successful adhesion of Cu2+ ions to PDA nanospheres with a total Cu2+ content of 16.5 wt%. The samples exhibited interconnected porous structures, increasing JNPs concentration resulting in larger pore size and higher porosity. The addition of JNPs to 10 % (Ch-JNP 10) resulted in the highest strength, young modulus, and crystallinity, while a reverse trend was observed at higher JNPs content. JNPs improve the antibacterial activity of chitosan-based dressing, especially against E. coli. All samples were biocompatible and did not exhibit any cytotoxic effects. Ch-JNP10 had higher cellular density, confluency, and collagen secretion than other samples. The in vivo study demonstrated that Ch-JNP10 induced epithelialization and oriented collagen fiber formation while reducing inflammation. Overall, Ch-JNP10 may be a potential wound dressing for chronic wounds.

The effect of green synthesis of TiO2 nanoparticles/collagen/HA scaffold in bone regeneration: As an animal study.

BACKGROUND: The bone defects cannot heal by themselves when their range exceeds the critical size defect (CSD). In clinical treatment, significant bone defects are often caused by trauma, developmental deformity, tumour resection and infection. OBJECTIVES: The purpose of this study was to investigate the effect of green synthesis of TiO2 from propolis extract/collagen/HA (Hydroxyapatite) scaffolds on bone regeneration in rats. METHODS: Water uptake, biodegradability, porosity and biodegradation of the scaffolds were evaluated after they were synthesised using freeze-dry method. Cell viability by MTT assay was then evaluated. During the 4, 8 and 12 weeks following the scaffold implantation, the bone regeneration was evaluated using macroscopic and microscopic tests to determine the effectiveness of green synthesis of TiO2 from propolis extract/collagen/HA scaffolds. RESULTS: Compared to the HA/Coll scaffold, ProTiO2 /HA/Coll scaffold was reduced porosity, water absorption and degradability porosity. Based on in vitro tests, both synthetic scaffolds induced cell growth and were less toxic and stimulated cell growth. Based on histopathological testing, the ProTiO2 /HA/Coll scaffolds formed high levels of bone during 12 weeks in comparison with HA/Coll and control group. CONCLUSIONS: ProTiO2 /HA/Coll composite can be used in regenerative medicine, bone fillers and scaffolds. As a result, this research suggests that ProTiO2 /HA/Coll composites could be promising candidates for bone regeneration.

Reinforcement of Calcium Phosphate Cement with Hybrid Silk Fibroin/Kappa-Carrageenan Nanofibers.

Calcium phosphate cements (CPCs) offer a promising solution for treating bone defects due to their osteoconductive, injectable, biocompatible, and bone replacement properties. However, their brittle nature restricts their utilization to non-load-bearing applications. In this study, the impact of hybrid silk fibroin (SF) and kappa-carrageenan (k-CG) nanofibers as reinforcements in CPC was investigated. The CPC composite was fabricated by incorporating electrospun nanofibers in 1, 3, and 5% volume fractions. The morphology, mineralization, mechanical properties, setting time, injectability, cell adhesion, and mineralization of the CPC composites were analyzed. The results demonstrated that the addition of the nanofibers improved the CPC mixture, leading to an increase in compressive strength (14.8 ± 0.3 MPa compared to 8.1 ± 0.4 MPa of the unreinforced CPC). Similar improvements were seen in the bending strength and work fracture (WOF). The MC3T3-E1 cell culture experiments indicated that cells attached well to the surfaces of all cement samples and tended to join their adjacent cells. Additionally, the CPC composites showed higher cell mineralization after a culture period of 14 days, indicating that the SF/k-CG combination has potential for applications as a CPC reinforcement and bone cell regeneration promoter.

The controlled release, bioactivity and osteogenic gene expression of Quercetin-loaded gelatin/tragacanth/ nano-hydroxyapatite bone tissue engineering scaffold.

In this study, a Gelatin/Tragacanth/Nano-hydroxyapatite scaffold was fabricated via freeze-drying method. A highly porous scaffold with an average pore diameter of 142 µm and porosity of 86% was found by the micro-computed tomography. The mean compressive strength of the scaffold was about 1.5 MPa, a value in the range of the spongy bone. The scaffold lost 10 wt.% of its initial weight after 28 days soaking in PBS that shows a fair degradation rate for a bone tissue engineering scaffold. Apatite formation ability of the scaffold was confirmed via scanning electron microscopy, X-ray diffraction and Fourier transforming infrared spectroscopy, after 28 days soaking in simulated body fluid. The scaffold was able to deliver 93% of the loaded drug, Quercetin, during 120 h in phosphate-buffered solution, in a sustainable manner. The MTT assay using human bone mesenchymal stem cells showed 84% cell viability of the Quercetin-loaded scaffold. The expression of the osteogenic genes including Col I, Runx-2, BGLAP (gene of osteocalcin), bFGF, SP7 (gene of osterix) and SPP1 (gene of osteopontin) were all upregulated when Quercetin was loaded on the scaffold, which indicates the synergetic effect of the drug and the scaffold.

Assessment of tricalcium phosphate/titanium dioxide (TCP/TiO2) nanocomposite scaffold compared with bone autograft and hydroxyapatite (HA) on the healing of segmental femur bone defect in rabbits.

Bone healing is a tissue process after a surgical operation. Many formulated materials have been designed for improving these procedures. The purpose of this study was to evaluate the effectiveness of nanocomposite tricalcium phosphate scaffolds combined with Titanium dioxide scaffold (TCP/TiO2) for femoral defects regeneration in rabbits. We studied 80 mature male New Zealand white rabbits weighing between 3 and 3.5 kg. Rabbits were subdivided into four groups. Anesthesia was performed before surgical operation by 50 mg/kg Ketamine 10% and 5 mg/kg xylazine 5% intramuscularly. We inducted a 6 × 5 mm diameter cylinder defect on the femur. Animals were separated into four trial groups of 20 animals each. After defecting, the experimental groups include control, autograft, hydroxyapatite, and TCP/TiO2 (received pure nanocomposite TCP/TiO2 material). A pathologist evaluated the sections on days 15, 30, 45, and 60 after surgery. The improvement of new and lamellar bone formation was the best in the nanocomposite TCP/TiO2 group at various point times, especially 60 days after surgery. We found that TCP/TiO2 nanocomposite has a significant improving function in the remodeling of bone in the defect areas. Graphical abstract.

The Effect of Gracilaria Corticata and Scenedesmus Acuminates Extract Mixture on the Healing of Wounds Contaminated with Staphylococcus in the Rat Model.

Introduction: Wound healing processes are dependent on the severity of the trauma, invasion of opportunistic microorganisms, and inflammatory, immunological, and metabolic responses. We tried to show the ability of algae to inhibit wound infection, which can lead to proper wound healing. Methods: Eighty rats were housed according to laboratory animal care protocols and divided into four groups at each operating time. Group I consisted of the non-treated animals. Group II was treated with 25% zinc oxide as a choice treatment. In the treated groups 3 and 4, an equal ratio of Gracilaria Corticata and Scenedesmus acuminate marine algae (mixed algae) was applied as 3% and 7% ointment pomade. Percentage of wound closure, number of bacteria in the wound surface, angiogenesis (Vascular endothelial growth factor; VEGF), the number of macrophages, collagen production level and transforming growth factor-beta (TGFß), epithelialization, and fibrosis were evaluated. Results: Applying mixed algae extract 7% and zinc oxide 25% could result in a mild improvement in wound closure (df: 9, 48; F=5.97; p<0.0001). In addition, mixed algae 3%, mixed algae 7% and zinc oxide could reduce the rate of bacterial growth compared to non-treated animals (df: 3, 16; F=5.74; p=0.0007). However, these improvements do not seem to be clinically significant. Induction of angiogenesis, increase in macrophage infiltration rate, and expression of TGFß are possible underlying mechanisms of mixed algae in accelerating wound healing process. Conclusion: The result showed that the administration of 3% and 7% mixed algae could mildly accelerate the wound healing process in a rat model of pelleted skin wound. However, it seems that its effect is not clinically significant compared to non-treated and zinc oxide treated animals.

Electrospun Silk Fibroin/kappa-Carrageenan Hybrid Nanofibers with Enhanced Osteogenic Properties for Bone Regeneration Applications.

In this study, a novel nanofibrous hybrid scaffold based on silk fibroin (SF) and different weight ratios of kappa-carrageenan (k-CG) (1, 3, and 5 mg of k-CG in 1 mL of 12 wt% SF solution) was prepared using electrospinning and genipin (GP) as a crosslinker. The presence of k-CG in SF nanofibers was analyzed and confirmed using Fourier transform infrared spectroscopy (FTIR). In addition, X-ray diffraction (XRD) analysis confirmed that GP could cause SF conformation to shift from random coils or α-helices to ß-sheets and thereby facilitate a more crystalline and stable structure. The ultimate tensile strength (UTS) and Young's modulus of the SF mats were enhanced after crosslinking with GP from 3.91 ± 0.2 MPa to 8.50 ± 0.3 MPa and from 9.17 ± 0.3 MPa to 31.2 ± 1.2 MP, respectively. Notably, while the mean fiber diameter, wettability, and biodegradation rate of the SF nanofibers increased with increasing k-CG content, a decreasing effect was determined in terms of UTS and Young's modulus. Additionally, better cell viability and proliferation were observed on hybrid scaffolds with the highest k-CG content. Osteogenic differentiation was determined from alkaline phosphatase (ALP) activity and Alizarin Red staining and expression of osteogenic marker genes. To this end, we noticed that k-CG enhanced ALP activity, calcium deposition, and expression of osteogenic genes on the hybrid scaffolds. Overall, hybridization of SF and k-CG can introduce a promising scaffold for bone regeneration; however, more biological evaluations are required.

Protective Effects of Phoenixin-14 Peptide in the Indomethacin-Induced Duodenal Ulcer: An Experimental Study.

Phoenixin-14 (PNX -14 ) is a newly identified neuropeptide with potential anti-inflammatory effects in the gastrointestinal tract. In this study, we evaluated the protective effect of PNX-14 against the formation of experimental indomethacin (IND)-induced duodenal ulcer. Thirty-two male Sprague-Dawley rats were randomly assigned to the four following study groups: (1) negative control (2) IND (7.5 mg/kg subcutaneous IND), (3) famotidine (FA) (7.5 mg/kg subcutaneous IND followed by 40 mg/kg intraperitoneal FA), and (4) PNX-14 (7.5 mg/kg subcutaneous IND followed by 50 µ/kg intraperitoneal PNX-14). Outcome measures included macroscopic evaluation of duodenal lesion, serum levels of IL-1ß, TNF-α, IL-6, and IL-12, and tissue biochemical parameters of oxidative stress, including malondialdehyde (MDA) , myeloperoxidase (MPO) activity, superoxide dismutase (SOD) activity, and catalase activity. Results The macroscopic grade of duodenal lesions were significantly smaller in the PNX-14 group than in the IND group (p < 0.001). Serum inflammatory cytokines were significantly increased in the IND group. PNX-14 treatment significantly decreased the serum levels of inflammatory cytokines (p < 0.0001). Oxidative contents (MDA and MPO activity) were significantly smaller in the PNX-14 group compared with the IND group (p < 0.0001), while anti-oxidative contents (SOD and catalase activity) were significantly more (p < 0.0001). PNX-14 was superior to FA in several anti-inflammatory properties, such as inhibiting the release of inflammatory cytokines and increasing the catalase activity. PNX-14 showed significant protective effects against the formation of IND-induced duodenal ulcers. These results suggest a promising therapeutic implication for PNX-14 in the treatment of gastrointestinal inflammatory disorders.

Wound closure, angiogenesis and antibacterial behaviors of tetracalcium phosphate/hydroxyethyl cellulose/hyaluronic acid/gelatin composite dermal scaffolds.

Polymeric and tetracalcium phosphate (TTCP)-containing polymeric scaffolds were fabricated using a freeze-drying technique, with a homogenous solution of hydroxyethyl cellulose (HEC)/hyaluronic acid (HA)/gelatin (G) or suspension of 15 or 20% TTCP) particles in HEC/HA/G solution. The morphology, phase composition, chemical bands, and swelling behavior of the scaffold were determined. In vitro fibroblast cell viability and migration potential of the scaffolds were determined by MTT, live/dead staining, and scratch assay for wound healing. The in vivo chick embryo angiogenesis test was also carried out. Finally, the initial antibacterial activity of the scaffolds was determined using Staphylococcus aureus. The scaffolds exhibited an enormous porous structure in which the size of pores increased by the presence of TTCP particles. While the polymeric scaffold was amorphous, the formation of low crystalline hydroxyapatite phase and the initial TTCP particles was determined in the composition of TTCP-added scaffolds. TTCP increased swelling behavior of the polymeric scaffold in PBS. The results demonstrated that the amount of TTCP was a crucial factor in cell life. A high concentration of TTCP could restrict cell viability, although all the scaffolds were nontoxic. The scratch assessments determined better cell migration and wound closure in treating with TTCP-containing scaffolds so that after 24 h, a wound closure of 100% was observed. Furthermore, TTCP-incorporated scaffolds significantly improved the angiogenesis, in the chick embryo test. The presence of TTCP had a significant effect on reducing the bacterial activity and 20% TTCP-containing scaffold exhibited better antibacterial activity than the others.

Bioorthogonal hydroxyethyl cellulose-based scaffold crosslinked via click chemistry for cartilage tissue engineering applications.

In this study, azide and alkyne moieties were introduced to the structure of citric acid-modified hydroxyethyl cellulose (HEC) and then through a bioorthogonal click chemistry method: Strain-promoted azide-alkyne cycloaddition, a novel crosslinked HEC scaffold (click sample) was obtained. Chemical modifications and successful crosslinking of the samples were assessed with FTIR and 1H NMR spectroscopy. Lyophilized samples exhibited a porous interconnected microarchitecture with desirable features for commensurate cartilage tissue engineering applications. As the stability of scaffolds improved upon crosslinking, considerable water uptake and swelling degree of ~650% could still be measured for the click sample. Offering Young's modulus of ~10 MPa and tensile strength of ~0.43 MPa, the mechanical characteristics of click sample were comparable with those of normal cartilage tissue. Various in vitro biological assays, including MTT analysis, cellular attachment, histological staining with safranin O, and real-time PCR decisively approved significant biocompatibility, chondrogenic ability, and bioorthogonal features of click sample.

In vitro evaluation of curcumin-loaded chitosan-coated hydroxyapatite nanocarriers as a potential system for effective treatment of cancer.

Nanotechnology has many potential applications in cancer treatment. For example, nano-drug delivery systems (NDDS) with high bioavailability, biodegradability, and biocompatibility have been developed, in order to increase the therapeutic effects of anticancer drugs. Among these NDDS, high-performance hydroxyapatite (HA) nanoparticles are rapidly advancing in the targeted cancer treatment due to their numerous benefits. Curcumin is an herbal metabolite that acts as a chemical inhibitor through the inhibition of tumor cells and the progression of many cancers. However, the poor bioavailability of curcumin is the most important challenge in using this substance. In this study, HA nanoparticles coated by chitosan were used as a pH-sensitive biopolymer to improve the efficiency and bioavailability of curcumin. For this purpose, HA nanoparticles were first synthesized by the sol-gel method. Then, a layer of chitosan was coated on it, and the curcumin drug was encapsulated in the nanocarrier, under controlled conditions. Techniques such as scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR) were used to characterize the nanocarriers. In the second part, nano-drugs prepared by various bioassays were examined. For this purpose, the rate of cytotoxicity by the methyl-thiazol-tetrazolium (MTT) assay and the rate of apoptosis induction by the acridine orange and ethidium bromide (AO/EB) staining method on the brain carcinoma U87MG cell line were investigated.

The synergistic effects of SrF2 nanoparticles, YSZ nanoparticles, and poly-ε-l-lysin on physicomechanical, ion release, and antibacterial-cellular behavior of the flowable dental composites.

Resin-based pit-and-fissure sealants (flowable resin composites) were formulated using bisphenol-A-glycerolatedimethacrylate (Bis-GMA)-triethylene glycol dimethacrylate-(TEGDMA)-diurethanedimethacrylate (UDMA) mixed monomers and multiple fillers, including synthetic strontium fluoride (SrF2) nanoparticles as a fluoride-releasing and antibacterial agent, yttria-stabilized zirconia (YSZ) nanoparticles as an auxiliary filler, and poly-ε-l-lysin (ε-PL) as an auxiliary antibacterial agent. Based on the physical, mechanical and initial antibacterial properties, the formulated nano-sealant containing 5 wt% SrF2, 5 wt% YSZ and 0.5 wt% ε-PL was selected as the optimal specimen and examined for ion release and cytotoxicity. The results showed an average release rate of 0.87 µg·cm-2·day-1 in the aqueous medium (pH 6.9) and 1.58 µg·cm-2·day-1 in acidic medium (pH 4.0). The maximum cytotoxicity of 20% toward human bone marrow mesenchymal stem cells (hMSCs) was observed according to the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) cytotoxicity assay and acridine orange staining test. A synergy between SrF2 nanoparticles and ε-PL exhibited a better antibacterial activity in terms of colony reduction compared to the other samples. However, the inclusion of SrF2 and ε-PL caused mechanically weakening of the sealants that was partly compensated by incorporation of YSZ nanoparticles (up to 10 wt%).

Effect of synthetic amorphous calcium phosphate nanoparticles on the physicochemical and biological properties of resin-modified glass ionomer cements.

The aim of this study was to find an optimum dose of the synthetic amorphous calcium phosphate (ACP) nanoparticles to be incorporated in resin-modified glass ionomer cements (RMGICs) for triggering the release of PO43-/Ca2+, alkaline phosphatase (ALP) activity and osteogenic differentiation of mesenchymal stem cells (hMSCs) without significantly affecting the essential properties of the cements. RMGICs were formulated from the powder composed of melt-derived strontium fluoro-aluminosilicate glass (SFAG) and synthetic ACP nanoparticles (0-20 wt%), as well as commercial polyalkenoic acid liquid. The effect of ACP incorporation on the workability, microstructure, Ca2+/PO43-/F- ion release and compressive strength was investigated. The response of hMSCs to the optimized cements was assessed by MTT cytotoxicity, ALP activity, and staining tests. The working time of the formulated RMGICs decreased significantly upon increase of ACP content from 5 to 20%. ACP (5%)-incorporated RMGICs showed improved photopolymerization and setting. An insignificant reduction was recorded in the compressive strength of RMGICs with addition of 1.5-5% ACP. The fluoride release didn't significantly decrease due to addition of 5% ACP. Upon incorporating 5% ACP, the biocompatibility of RMGICs rose to about 20%. In addition, ALP activity and osteogenic differentiation of hMSCs noticeably increased after exposure to ACP-incorporated RMGIC. ACP (5%)-incorporated RMGICs could be promising candidates for both restorative and regenerative dentistry owing to the optimum mechanical strength, prolonged ion release, and their effective role in the cell differentiation and biomineralization demanded for pulp regeneration.

Creep behavior of Biodegradable Triple-component Nanocomposites Based on PLA/PCL/bioactive Glass for ACL Interference Screws.

BACKGROUND: Short-time creep behaviorfor aseries of biodegradable nanocomposites, which areused as implantable devices inthe body, is a crucial factor.The present study aimed to investigate the effect of bioactive glass nanoparticles (BGn) on creep and creep-recovery behaviors of polylactic acid/polycaprolactone (PLA/PCL) blends at different given loads and different applied temperatures. METHODS: A series of biodegradable nanocomposites consisted of PLA/PCL blends (comprising 80 parts PLA and 20 parts PCL) with different amounts of modified-BGn (m-BGn) fillers were prepared using the evaporated solvent casting technique. Creep and creep-recovery behaviors of all specimens were studied at different valuable stressesof 3 and 6 MPa and different given temperatures of 25 and 37°C. RESULTS: In all cases, m-BGn improved the creep resistance of the nanocomposites due to the retardation effect during the creep behaviors of the nanocomposite systems. The obtained results in terms of creep and creep-recovery properties determined that the nanocomposites of PLA/PCL/m-BGn can satisfy the required conditions of an appropriate anterior cruciate ligament reconstruction (ACL-R) screw. CONCLUSION: The obtained results confirmed that the BGn plays an impeding role in the movement of PLA/PCL chains leading to in increase the creep resistance. According to the results, it was determined that the nanocomposites of PLA/PCL and m-BGn can satisfy the required circumstances of a proper ACL-R screw.

Histopathological Evaluation of Polycaprolactone Nanocomposite Compared with Tricalcium Phosphate in Bone Healing.

INTRODUCTION: In recent years, the use of bone scaffolds as bone tissue substitutes, especially the use of such as hydroxyapatite and tricalcium phosphate, has been very popular. Today, the use of modern engineering techniques and advances in nanotechnology have expanded the use of nanomaterials as bone scaffolds for bone tissue applications. MATERIAL AND METHODS: This study was performed on 60 adult male New Zealand rabbits divided into four experimental groups: the control group without any treatment, the second group receiving hydroxyapatite, the third group treated with ß-tricalcium phosphate, and the fourth group receiving nanocomposite polycaprolactone (PCL) scaffold. In a surgical procedure, a defect 6 mm in diameter was made in a hind limb femur. Four indexes were used to assess histopathology, which were union index, spongiosa index, cortex index, and bone marrow. RESULTS: The results showed that nanocomposite PCL and control groups always had the respective highest and lowest values among all the groups at all time intervals. The histopathological assessment demonstrated that the quantity of newly formed lamellar bone in the nanocomposite PCL group was higher than in other groups. CONCLUSION: All these data suggest that PCL had positive effects on the bone healing process, which could have great potential in tissue engineering and clinical applications.

Assessment of tricalcium phosphate/collagen (TCP/collagene)nanocomposite scaffold compared with hydroxyapatite (HA) on healing of segmental femur bone defect in rabbits.

Bone regeneration is an important objective in clinical practice and has been used for different applications. The aim of this study was to evaluate the effectiveness of nanocomposite tricalcium phosphate (TCP)/collagen scaffolds combined with hydroxyapatite scaffold for bone healing in surgery of femoral defects in rabbits. In this study, 45 mature male New Zealand white rabbits between 6 and 8 months old and weighting between 3 and 3.5 kg were examined. Rabbits were divided into three groups. Surgical procedures were performed after intramuscular injection of Ketamine 10% (ketamine hydrochloride, 50 mg/kg) and Rompun 5% (xylazine, 5 mg/kg). Then an approximately 6 mm diameter-5 mm cylinder bone defect was created in the femur of one of the hind limbs. After inducing the surgical wound, all rabbits were coloured and randomly divided into three experimental groups of 15 animals each. Group 1 received pure medical nanocomposite TCP/collagen granules, group 2 received hydroxyapatite, and third group was a control group which received no treatment. Histopathological evaluation was performed on days 15, 30, and 45 after surgery. On days 15, 30, and 45 after surgery, the quantity and the velocity of stages of bone formation at the healing site in nanocomposite TCP/collagen group were better than HA and control groups and the quantity of newly formed lamellar bone at the healing site in nanocomposite TCP/collagen group were better than onward compared with HA and control groups. In conclusion, it seems that TCP/collagen nanocomposite has a significant role in the reconstruction of bone defects and can be used as scaffold in bone fractures.

Poly (d/l) lactide/polycaprolactone/bioactive glasss nanocomposites materials for anterior cruciate ligament reconstruction screws: The effect of glass surface functionalization on mechanical properties and cell behaviors.

In this paper, different nanocomposites made of a polymer blend (80% of PDLLA and 20% of PCL in w/w) and various amounts of a sol-gel derived bioactive glass nanoparticles (0, 1, 3 and 6wt%) were prepared using a solvent-evaporation technique. The morphology, mechanical properties and osteoblastic cell behaviors of the nanocomposites were evaluated. According to the early results, addition of bioactive glass nanoparticles to the polymer matrix reduced the tensile and flexural strength because of a non-uniform distribution of the nanoparticles. Thus, a homogeneous dispersion was obtained by surface modification of the glass nanoparticles using (3-aminopropyl)triethoxysilane as a coupling agent. The results showed that the tensile and flexural strength of the nanocomposite were improved by the nanoparticle functionalization, however the glass content was a crucial factor. The maximum tensile and flexural strength values of 38MPa and 94MPa were obtained for the polymer matrix loaded with 3wt% of the modified nanofiller and further increase of filler content led to sever agglomeration and hence a reduction of the mechanical properties. The obtained mechanical properties are favorable for anterior cruciate ligament reconstruction screws. Besides, the results of cell culture using human osteoblastic cells illustrated better cell attachment and cell growth of the nanocomposites compared to the neat polymer blend.